INSTITUTE OF CYTOLOGY OF THE RUSSIAN ACADEMY OF SCIENCES

Laboratory of Cellular Pathology

    Head of the Laboratory

    KUDRYAVTSEV Boris Nikolaevich

     DSc., PhD, Professor

    Tel.: +7 (812) 297-37-96
    e-mail: cellpath@mail.cytspb.rssi.ru

    Laboratory was established in 1994.

The object of reseach -vertebrate hepatocytes and cardiomyocytes, trophoblast cells, human and animal cell lines. Novel data were obtained in the lab on the growth mechanisms of the normal and pathologically modified liver; mechanisms of polyploidization of hepatocytes, cardiomyocytes and trophoblasts; glycogen metabolism and structure in normal and pathologically modified human and animal liver; and mechanisms of cell cycle regulation and progenitor cell differentiation into mature endodermal cells.



Laboratory personnel

Zybina T.G., DSc, PhD, principal research scientist
Stein G.I., DSc, PhD, principal research scientist
Popov B.V., DSc, MD, leading research scientist
Sakuta G.A., PhD, senior research associate
Bezborodkina N.N., PhD, senior research associate
Bayduk E.V., PhD, research associate
Petrov N.S., PhD, research associate
Chestnova A.Yu., junior research associate

Research interests:

Current methods of cellular and molecular biology, biochemistry, polarography and cytogenetics are employed in the lab. Methods of analytical microscopy, such as absorption cytophotometry, cytofluorimetry, microinterferometry, confocal microscopy, image analysis and others are also widely used in the lab. The main focus of investigation is the study of the kinetics and morpho-functional changes in the cells during development and regeneration of normal and pathologically modified mammalian organs.

    There are several ongoing projects in the laboratory:
  • Investigation of the role of cellular heterogeneity in sustaining the cell functions of normal and pathologically modified organs (i. e. liver, heart, trophoblast).
  • Investigation of the regeneration potential and the cellular mechanisms of regeneration of pathologically altered organs (liver, heart).
  • Studies of the carbohydrate metabolism in normal and pathologically modified liver specimens, including chronic hepatitis and cirrhosis of various etiology.
  • Investigation of the biological role of cellular polyploidy and the functional properties and mechanisms of polyploidal cell formation.
  • Assessment of the mitochondrial respiratory chain functions in normal and pathologically modified hepatocytes.
  • Research on molecular mechanisms of stem cell differentiation into hepatocytes and on other cells of endodermal derivation. Also, purification of diagnostic and clinically relevant monoclonal antibodies against characteristic markers of stem and differentiated cells.

5 doctorse and 16 candidats dissertations have been defended in the lab.


Publications

Agafonova N. A., GrinchukT. M., Korablev V. P., Rozanov Yu. M., Sakuta G.A., Shilina M.A., Shtein G.I., Kudruavtsev B.N. 2015. DNA content of individual chromosomes of Chinese hamster normal karyotype and its permanent cell lines V-79 RJK and Verb-5. Cell and Tissue Biology, 9(5): 371-376.

Chestnova A.Yu., Bezborodkina N.N., Matyukhina N.M., Kudryavtsev B.N. Dynamics of proglycogen and macroglycogen in hepatocytes of normal and cirrhotic rat liver at various stages of glycogenesis. 2015. Cell and Tissue Biology, 9(2): 133-140.

Baidyuk E.V., Sakuta G.A., Kislyakova L. P., Kislyakov Yu. Ya. , Kudryavtsev B.N., Okovity S.V. 2015. Rat heart structural and functional characteristics and gas exchange parameters after experimental myocardial infarction. Cell and Tissue Biology, 9(1): 24-29.

Bezborodkina N.N., Chestnova A.Yu., Okovity S.V., Kudryavtsev B.N. 2014. Activity of glycogen synthase and glycogen phosphorylase in normal and cirrhotic rat liver during glycogen synthesis from glucose or fructose. Exp Toxicol Pathol. 66:147-154.

Zybina T.G., Stein G.I., Pozharisski K.M., Zybina E.V. 2014. Invasion and genome reproduction of the trophoblast cells of placenta junctional zone in the field vole, Microtus rossiaemeridionalis. Cell Biol Int. 38:136-43.

Zybina T.G., Zybina E.V. 2014. Genome variation in the trophoblast cell lifespan: diploidy, polyteny, depolytenization, genome segregation. World J Med Genet. 4 (4):77-93.

Baidyuk E.V., Korshak O.V., Karpov A.A., Kudryavtsev B.N., Sakuta G.A. 2013. Cellular Mechanisms of Rat Liver Regeneration after Experimental Myocardial Infarction. Cell and Tissue Biology, 7(2):140-148.

Bezborodkina N.N., Okovity S.V., Chestnova A.Yu., Kudryavtsev B.N. 2013. Hepatocytes of cirrhotic rat liver accumulate glycogen more slowly than normal ones. Hepatol Int. 7(4): 1084-1090.

Bezborodkina N.N., Chestnova A.Yu., Kudryavtsev B.N. 2013. Glycogen metabolism during chronic liver diseases. In: P.L. Weiss and B. D. Faulkner, editors. Glycogen: Structure, Functions in the Body and Role in Disease. NOVA Publishers. P. 47-80. ISBN: 978-1-62948-395-5.

Agafonova N. A., Sakuta G.A., Rozanov Yu. M., Shtein G.I., Kudruavtsev B.N. 2013. DNA image-fluorimetry of individual human chromosomes. Cell and Tissue Biology, 7(4):352-361.

Bezborodkina N.N., Stein G.I., Sivova E.V., Chestnova A.Yu., Kudryavtsev B.N. 2011. Analysis of structure of glycogen in rat hepatocytes using cytochemical and FRET methods. Cell and Tissue Biology, 5(5):417-427.

Zybina T.G., Stein G.I., Zybina E.V. 2011. Endopolyploid and proliferating trophoblast cells express different patterns of intracellular cytokeratin and glycogen localization in the rat placenta. Cell Biol. Int. 35(7): 649-655.

Okovity S. N., Bezborodkina N. N., Uleichik S.G., Shulenin S.N. 2010. Hepatoprotectors. GEOTAR-Media. Moscow. 112 p. (In Russian).

Bezborodkina N. N., Kirshina E. I., Mushinskaya E. V., Kudryavtsev B. N. 2009. Application of Schiff s reagents with various spectral characteristics to determine the content of labile and stable glycogen fractions in isolated hepatocytes. Tsitologiya. v. 51 (12), pp. 1025-1035. (In Russian).

Shiryaeva A., Arkadyeva A., Emelyanova L., Sakuta G., Morozov V. 2009. Superoxide anion production by the mitochondrial respiratory chain of hepatocytes of rats with experimental toxic hepatitis. J. Bioenerg. Biomembr., v. 41, pp. 379-385.

Baidyuk E. V., Shiryaeva A. P., Bezborodkina N. N., Sakuta G. A. 2009. Analysis of functioning and morphology of primary hepatocytes of rats with experimental toxoc hepatitis. Tsitologiya. v. 51 (10), pp. 797-805. (In Russian).

Bezborodkina N. N., Vakhtina A. A., Baidyuk E. V., Yakupova G. S., Kudryavtsev B. N. 2009. Interrelations between the glycogen content in hepatocytes and their size in normal and cirrhotic rat liver. Tsitologiya. v. 51 (5), pp. 417-427. (In Russian).

Zybina E. V., Bogdanova M.S., Stein G.I., Vlasova T.D., Zybina T. G. 2009. Endopolyploidization and the interstitial invasion of the supergiant trophoblast cells of the field vole Microtus rossiaemeridionalis. Tissue & Cell, v.41, pp. 361-366.

Shiryaeva A., Baidyuk E., Arkadieva A., Okovity S., Morozov V. and Sakuta G. 2008. Hepatocyte mitochondrion electron-transport chain alterations in CCl4 and alcohol induced hepatitis in rats and their correction with simvastatin. J. Bioenerget. Biomembr. 2008. v. 40, ננ. 27-34.

Zybina E. V., Zybina T. G. 2008. Modifications of nuclear envelope during differentiation and depolyploidization of rat trophoblast cells. Micron, v. 39, pp. 593-606.

Bezborodkina N. N., Okovity S. N., Kudryavtsev B. N. 2008. Carbohydrates metabolism at the chronic liver diseases. Saint Piterpsburg. Sintez-Buk. 176 p. (In Russian).

Zybina E.V., Zybina T.G., Bogdanova M.S., Stein G.I. 2005. Whole-genome chromosome distribution during nuclear fragmentation of giant trophoblast cells of Microtus rossiaemeridionalis studied with the use of gonosomal chromatin arrangement. Cell Biol. Internat., v. 29, pp. 1066-1070.

Sakuta G. A., Kudryavtsev B. N. Cellular mechanisms of cirrhotic rat liver regeneration. II. Proliferation, polyploidization and hypertrophy after partial hepatectomy. Tsitologiya. 2005. 47 (5): 379-387. (In Russian).

Bezborodkina N. N., Kudryavtseva M. V., Okovity S. N., Nilova V. K., Kudryavtsev B. N. 2003. Glycogen dynamics in normal and cirrhotically altered liver following glucose administration to starving rats. Tsitologiya. v. 45 (10), pp. 1019-1026. (In Russian).

Kudryavtseva M.V., Bezborodkina N.N., Radchenko V.G., Okovity S.V., Kudryavtsev B.N. 2001. Metabolic heterogeneity of glycogen in hepatocytes of patients with liver cirrhosis: the glycogen content of the liver lobule zones in cirrhosis. Europ. J. Gastroenterol. Hepatol., v. 13, pp. 693-697.

Kudryavtseva M.V., Bezborodkina N.N., Kudryavtsev B.N. 2001. Glycogen-forming function of hepatocytes in cirrhotically altered rat liver after treatment with chorionic gonadotropin. Exp. Toxic Pathol., v. 53, pp. 57-63.

Kudryavtseva M.V., Bezborodkina N.N., Kudryavtsev B.N. 1999. Restoration of the glycogen-forming function of hepatocytes in rats with liver cirrhosis is faciliated by high-carbohydrate diet. Brit. J. Nutrit., v. 81, pp. 473-480.

Kudryavtsev B. N., Anatskaya O.V., Nilova V.K., Komarov S.A. 1997. Intrrelation between myofibrillar and mitochondrial apparatus and cardiomyocyte polyploidy and hypertrophy in mammals with differtent body weight. Tsitologiya. v. 39 (10), pp. 946-964. (In Russian).

Kudryavtseva M.V., Sakuta G.A., Skorina A.D., Stein G.I., Emelyanov A.V., Kudryavtsev B.N. 1996. Quantitative analysis of glycogen content in hepatocytes of portal and central lobule zones of normal human liver and in patients with chronic hepatitis of different ethiology. Tissue & Cell, 28 (3) 279-285.

Sakuta G. A., Kudryavtsev B. N. 1996. Cellular mechanisms of cirrhotic rat liver regeneration. I. Interrelation between cell proliferation, polyploidization and hypertrophy after chronic CCl4-treatment. Tsitologiya. v. 38 (11), pp. 1019-1026. (In Russian).

Kudryavtsev B.N., Kudryavtseva M.V., Sakuta G.A., Stein G.I. 1993. Human hepatocyte polyploidization kinetics in the course of life cycle. Virchows Archiv B cell Pathol., v. 64, pp. 387-393.

Stein G., Kudryavtsev B. 1992. A method for investigation hepatocyte polyploidization kinetics during postnatal development in mammals. J. Theor. Biol., v.156, pp. 349-363.

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