Amoebae Cultures Collection of Institute of Cytology – ACCIC(SPb) Laboratory of Cytology of Unicellular Organisms

STRAINS (CLONES) OF AMOEBA PROTEUS AND AMOEBA SP. (PROTEUS-LIKE), RECEIVED FROM DIFFERENT LABORATORIES

Amoeba proteus (Pallas 1766) Leidy 1878, strain B From M. Müller (Medical University, Budapest, Hungary) in 1959. Established at King's College (London, UK) in 1950th.

Amoeba proteus (Pallas 1766) Leidy 1878, strain Da From M.J. Ord (Southampton University, Great Britain) in 1970. Established by J.A. Dawson from Floral Park (New York, USA) no later than 1950.

Amoeba proteus (Pallas 1766) Leidy 1878, strain Bk From D.M. Prescott (University of Colorado, Boulder, USA) in 1969. Established at Zoological Department, University of California (Berkeley, USA) in 1952.

Amoeba proteus (Pallas 1766) Leidy 1878, strain F From M. Tuffreau (Laboratoire de Zoologie II, Orsay, France) in 1971.

Amoeba proteus (Pallas 1766) Leidy 1878, strain MGU From Moscow State University, (Moscow, Russia) in 1960. Isolated from nature in Moscow province.

Amoeba proteus (Pallas 1766) Leidy 1878, strain A From M. Taylor (Notre Dame Training College, Glasgow, Scotland) in 1964. Notes. According to the personal communication M. Taylor, this strain was established in 1918.

Amoeba proteus (Pallas 1766) Leidy 1878, strain C From M.M. Isakova-Keo (Department of Invertebrate Zoology, Leningrad State University) in 1964. Established by A.V. Yankowsky, isolated from pound located in Mozhaiskaya village (St.Petersburg province) in 1962.

Amoeba proteus (Pallas 1766) Leidy 1878, strain D From M.J. Ord (Southampton University, Great Britain) in 1983. Notes. Initially amoebae of this strain was identified as Amoeba discoides Shaeffer, 1916, subsequently this species was synonymised with A. proteus (Jeon and Lorch, 1973).

Amoeba proteus (Pallas 1766) Leidy 1878, strain T1D From M. Müller (Medical University, Budapest, Hungary) in 1963. Established at King's College (London, UK) in 1948. Notes. Initially amoebae of this strain was identified as Amoeba discoides Shaeffer, 1916, subsequently this species was synonymised with A. proteus (Jeon and Lorch, 1973).

Amoeba proteus (Pallas 1766) Leidy 1878, strain T1P From M.J. Ord (Southampton University, Great Britain) in 1975. Established by M. Taylor (Notre Dame Training College, Glasgow, Scotland) in 1948.

Amoeba proteus (Pallas 1766) Leidy 1878, strain POL From L.N. Seravin (Biological Research Institute, Leningrad State University) in 1962. This strain was brought by L.N. Seravin from Poznan (Poland) where it was cultivated in one of protozoological laboratories.

Amoeba proteus (Pallas 1766) Leidy 1878, strain CCAP LB 1503/4 From F.C. Page (Institute of Terrestrial Ecology, Cambridge University, UK) in 1984 . This strain was deposited in Culture Collection of Algae and Protozoa (CCAP).

Amoeba proteus (Pallas 1766) Leidy 1878, штамм CCAP LB 1503/8 From F.C. Page (Institute of Terrestrial Ecology, Cambridge University, UK) in 1991. This strain was deposited in Culture Collection of Algae and Protozoa (CCAP).

Amoeba proteus (Pallas 1766) Leidy 1878, штамм CCAP PADPY From F.C. Page (Institute of Terrestrial Ecology, Cambridge University, UK) in 1991. This strain was deposited in Culture Collection of Algae and Protozoa (CCAP).

Amoeba sp., strain BY13 (proteus-like) From D.V. Ossipov (Biological Research Institute, Leningrad State University) in 1989. Isolated from pound in Borok settlement (Yaroslavl province, Russia).

Amoeba sp., strain Neapol (proteus-like) From S.I. Fokin (Department of Invertebrate Zoology, St.Petersburg State University, Russia) in 2005 as natural isolate; cloned. Isolated from the natural reservoir at Neapol vicinities (Italy).

Amoeba proteus (Pallas 1766) Leidy 1878, strain Dz Established by M.V. Tavrovskaya (Institute of Cytology RAS, St. Petersburg, Russia) in 1984. Isolated from pound located in Lopuhinsky Garden (St. Petersburg, Russia).

Amoeba proteus (Pallas 1766) Leidy 1878, strain Petrozavodsk Established by G.N. Nikolaeva (Institute of Cytology RAS, St. Petersburg, Russia) in 1968. Isolated from pound located in surroundings of Petrozavodsk (Republic of Karelia, Russia).

Amoeba sp., strain SHAPKI (proteus-like) Established by M.V. Vladimirov (Institute of Cytology RAS, St. Petersburg, Russia) in 1993. Isolated from pound in Shapki settlement (St.Petersburg province), october 1993.

Amoeba sp., strain CONT (proteus-like) Established by A.S. Karpov (Institute of Cytology RAS, St. Petersburg, Russia) in 1989. Isolated from Kontrol'nyi gulf, Lake Sys'jarvi (Valaam Archipelago, North-Western Russia).

Amoeba sp., strain VAL (proteus-like) Established by A.S. Karpov (Institute of Cytology RAS, St. Petersburg, Russia) in 1989. Isolated from Skitskyi gulf, Lake Sys'jarvi, (Valaam Archipelago, North-Western Russia), august 1989, bottom sediments near the coast line.

Amoeba sp., strain KAN (proteus-like) Established by A.S. Karpov (Institute of Cytology RAS, St. Petersburg, Russia) in 1989. Isolated from Lake Kanevsoe (Valaam Archipelago, North-Western Russia), september 1989, from microfouling community.

Amoeba sp., strain VSK (proteus-like) Established by A.S. Karpov (Institute of Cytology RAS, St. Petersburg, Russia) in 1990. Isolated from a fire reservoir, Voskresensky monastery (Valaam Archipelago, North-Western Russia), july 1990.

Amoeba sp., strain MURINO (proteus-like) Established by N.N. Bobyleva (Institute of Cytology RAS, St. Petersburg, Russia) in 1974. Isolated from a pound in Murino settlement (St.Petersburg province, Russia).

Amoeba sp., strain Olgino (proteus-like) Established by M.V. Tavrovskaya (Institute of Cytology RAS, St. Petersburg, Russia) in 2013. Isolated from Olginskyi pond (Svetlanovskyi ave., St.Petersburg, Russia), september 2013.

Amoeba proteus (Pallas 1766) Leidy 1878, strain C4 From L.V. Kalinina (Institute of Cytology RAS, St. Petersburg, Russia) in 1978. Established by L.B. Gorunova (Institute of Cytology RAS, St. Petersburg, Russia) after RNAse treatment of Amoeba proteus strain C.

Amoeba amazonas (Flickinger, 1974) Friz, 1992 (strain Amazon) From D.M. Prescott (University of Colorado, Boulder, USA) in 1969. Estabished by D.M. Prescott from river Amazon (Brazil). Notes. A long time the status of amoebae of this strain as a separate species was in doubt, because the name "was given without a description that is taxonomically valid..." (Page, 1988, p. 59); the formal taxonomical status of this species was established later (Friz, 1992).

Amoeba indica Rao 1971 (strain Indica) From F.C. Page (Institute of Terrestrial Ecology, Cambridge University, UK) in 1985. This strain was deposited in Culture Collection of Algae and Protozoa (CCAP). Established by M.V.N. Rao (Bombay, India) in 1971. Notes. Amoebae of this strain initially appear in literature as belonging to separate species A. indica (Rao, Chatterjee, 1974), however, this name was given without a description that is taxonomically valid (Page, 1988); the differences of these amoebae from other species of Amoeba genus indicated in some publications (Friz, 1983, 1987, 1992; Sopina, 1999; 2000).

Amoeba borokensis Kalinina, Afon'kin, Gromov, Khrebtukova et Page, 1986 (strain BOR) Established by L.V. Kalinina (Institute of Cytology RAS, St. Petersburg, Russia) in 1974. Isolated from pound in Borok settlement (Yaroslavl province, Russia), august 1974. Notes. It was initially established as a new strain of A. proteus, later was described as a separate species (Kalinina et al., 1986).

Amoebasp., strain BELOMOR Established by D.V. Gromov (Institute of Cytology RAS, St. Petersburg, Russia) in 1985. Isolated from the freshwater lake at Srednyi Island, Chupa Inlet (Kandalaksha Gulf, Western White Sea), august 1985. Notes. Amoebae of this strain differ from known species of the genus Amoeba (see: Sopina, 2000; Podlipaeva, Goodkov, 2009).

Amoebasp., strain DG Established by D.V. Gromov (Institute of Cytology RAS, St. Petersburg, Russia) in 1993. Isolated from the freshwater lake at Srednyi Island, Chupa Inlet (Kandalaksha Gulf, Western White Sea), july 1993. Notes. Amoebae of this strain differ from known species of the genus Amoeba and remind amoebae of the strain BELOMOR (presumably, they belong to the same species).

Trichamoeba sp., strain AS-102 From D.V. Ossipov (Biological Research Institute, Leningrad State University) in 1971. Isolated from a small stream in the Kyzylagachsky reserve (Azerbaijan), june 1971. Notes.