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CELL CULTURES
Information Bulletin, Issue 29, St. Petersburg, 2013. ISSN 2077-6055 |
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INFLUENCE OF ADIPOSE TISSUE MESENCHIMAL STROMAL CELLS ON THE PROLIFERATION OF LYMPHOBLASTOID LINE NAMALVA
Mesenchimal stromal cells (MSC) are able to inhibit or to stimulate the proliferation of transformed cells dependently on their histogenesis, differentiation level and/or culture conditions. The aim of the work was to investigate the influence of MSC from adipose tissue on the proliferation of lymphoblastoid cell line Namalva under different cultivation conditions. The cells of both types were maintained in the atmosphere containing 5% ÑÎ2 and 20% Î2 (normoxia) or 5% Î2 (hypoxia). The deficiency of growth factors in culture medium was created by using the serum with low growth-stimulating activity. Hypoxia stimulated the proliferation of Namalva cell in standard mediun but demonstrated inhibitory action upon growth factors deficiency. During cocultivation of Namalva cells with MSC the formation of close intercellular contacts between cells of both types was observed. The cocultivation with MSC in standard medium stimulated the proliferation of Namalva cells either in normoxia or in hypoxia. In growth factors-defficient medium the presence of MSC supported the survival and proliferation of lymphoblastoid cell in hypoxic and normoxic conditions. Thus, MSC from adipose tissue are able to support the survival and proliferation of Namalva cell line upon cultivation in growth factors-defficient culture medium. Key words: stem cells, lymphoblastoid lines, cocultivation POSSIBILITIES OF USE OF MESENCHYMAL STEM CELLS FROM A ADIPOSE TISSUE IN REGENERATIVE TREATMENT OF DISEASES CNS (THE LITERATURE REVIEW)
In the literature review the modern representations about biological properties of adult mesenchymal stem cells (MSC) an adult organism are summerized. The special attention is given to features of receiving and cultivation of the pluripotent autologous MSC of the adipose tissue stromal-vaskular fraction (ADSC). Methods of directed neurogenic differentiation ADSC and prospects of their use in regenerative treatment of the severe CNS diseases are considered. Possible undesirable consequences and dangers of an uncontrollable spontaneous differentiation of MSC in diverse cellular lines are highlighted. STEM AND PROGENITOR CELLS OF HUMAN OLFACTORY MUCOSA: CONDITIONS OF ISOLATION AND ACCUMULATION IN CULTURE, MORPHOFUNCTIONAL AND
PHENOTYPIC CHARACTERISTIC
Isolation and cultivation human of stem and progenitor cells from the olfactory mucosa (OM) is relevant direction for the development of treatment protocols for traumatic and degenerative lesions of the nervous system. In this study, conditions for isolation of viable cells from OM with high proliferative potential were optimized for the preparation of heterogeneous primary culture. Morphofunctional and phenotypic composition of cultured cells was characterized. Two populations of stem and progenitor cells were identified. Key words: human olfactory mucosa, cell culture, ecto-mesenchymal stem cells, cytospheres INFLUENCE OF DIFFERENT INFLUENZA A SUBTYPES ON PROLIFERATION AND TUMOR NECROSIS FACTOR INDUCTION (TNF) IN PNEUMOCYTE (A-549) AND
ENDOTHELIOCYTE (ECV-304) HUMAN CELL LINES IN PRESENCE OF ANTIVIRALS
The study of stimulating effect of low-dose influenza A infection on human monolayer cell cultures was continued. This time it was shown that endothelial cell line ECV-304 when infected responded by enhanced proliferation not only to pandemic influenza A (H1N1)pdm and A (H3N2) subtype, but also to A (H2N2) subtype but they didn't show any augmented proliferation in response to A (H5N1) subtype. In contrast to ECV-304 cell line, the A-549 cell culture showed enhanced proliferation only in response to A (H1N1)pdm and A(H3N2) influenza subtypes. Weak biological activity in A-549 cells was also observed studying the expression of tumor necrosis factor (TNF), one of the major proinflammatory cytokines which plays a crucial role in influenza A pathogenesis. No correlation was found between the influence of different antivirals on virus-induced proliferation and TNF expression: only rimantadine simultaneously inhibited virus-induced cell proliferation and TNF expression. These data demonstrate differential susceptibility of epithelial and endothelial cell cultures in response to low-dose viral infection and their distinct profile of TNF expression. The necessity of adoption of new criteria for evaluation of "virus-host cell" interactions for understanding mechanisms of action of new antivirals is being discussed. Key words: influenza A viruses, cell lines A-549, ECV-304, TNF expression, cell proliferation STUDY OF CYTOMORPHOLOGICAL CHANGES IN VERO-V CEII MONOLAYER DEPENDING ON THE PERIOD OF AVIAN METAPNEWMOVIRUS REPRODUCTION
The results of avian metapneumovirus interaction with Vero-V cells at different periods of virus cultivation are presented. Correlation between the character of cell monolayer infection and amount of the virus detected when titrating has been established. Key words: virus reproduction, avian metapneumovirus, Vero-V cells, syncytium, symplast INSTRUCTIONS TO AUTHORS The paper should not exceed 10 manuscript pages. The manuscript is to be accompanied by recommendation addressed to the
Editorial Board of the Information Bulletin from the institution in which the work has been carried out. The paper should contain a brief resume, with the tittle of the work,
names of the authors, institution and city.
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